Methods of quantitating heavy and light chain polypeptide pairs
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United States of America Patent
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Sep 26, 2017
Grant Date -
Jul 30, 2015
app pub date -
Oct 3, 2013
filing date -
Oct 3, 2012
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Abstract
Provided is a method of quantitatively determining the ability of individual IgG heavy chains to selectively pair with a particular IgG light chain when the heavy chains and two unique light chains are co-expressed. The method provides results with reasonable throughput and is robust and accurate. The co-expressed heavy and light chains do not need to be isolated and purified which enables more efficient screening.
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