Method for preparing protein cage, and in situ method for preparing hydrophobic additive-supported core-shell structured polymer-protein particles
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United States of America Patent
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Sep 12, 2017
Grant Date -
May 5, 2016
app pub date -
Oct 11, 2013
filing date -
Mar 22, 2013
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Abstract
The present invention relates to a method for preparing a protein cage which comprises: a 1st step of preparing an amphiphilic polymer comprising a 1st hydrophobic polymer and a 1st hydrophilic functional group; a 2nd step of preparing a hydrophilic protein comprising a 2nd functional group binding to the 1st functional group; a 3rd step of forming an amphiphilic polymer-protein hybrid by the binding of the 1st functional group and the 2nd functional group, and forming core-shell structured particles comprising a protein shell and an amphiphilic polymer core by the self-assembly of the amphiphilic polymer in a hydrophilic solvent; and a fourth step of removing some or all of the hydrophobic polymer of the core part from the core-shell structured particles.
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