Process for purifying proteins
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United States of America Patent
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Sep 5, 2017
Grant Date -
Jul 11, 2013
app pub date -
Jul 27, 2011
filing date -
Jul 27, 2010
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Abstract
The present invention provides method for purifying a recombinant protein from a gram-negative bacterial host cell sample or extract thereof wherein said host cell expresses a recombinant protein and a recombinant disulphide isomerase DsbC; comprising: a. adjusting the pH of the host cell sample or extract thereof to a pH of 5 or less to precipitate the recombinant disulphide isomerase; and b. separating precipitated recombinant disulphide isomerase DsbC from the recombinant protein to produce a recombinant protein sample.
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