Fluorescence receiving apparatus and fluorescence receiving method
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United States of America Patent
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Aug 22, 2017
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app pub date -
Mar 4, 2014
filing date -
Mar 6, 2013
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Abstract
A fluorescence receiving apparatus comprises an excitation light source, a spatial light modulator of a phase modulation type for phase-modulating excitation light to obtain phase-modulated light, a focusing optical system configured to focus the phase-modulated light to a specimen, a specimen stage for supporting the specimen, a fluorescence receiver for receiving fluorescence generated by focus of the phase-modulated light to the specimen, a control unit for displaying a first CGH on the spatial light modulator, and a correction unit for correcting the first CGH. The correction unit comprises a receiver-specific sensitivity information storage preliminarily acquiring and storing sensitivity information per reception position specific to the fluorescence receiver, and a second hologram generator for correcting the first CGH, based on intensities of the fluorescence and the sensitivity information, to generate a second CGH. The control unit displays the second CGH on the spatial light modulator.
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