plasmid DNA production
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United States of America Patent
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Aug 8, 2017
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app pub date -
Feb 3, 2015
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May 23, 2007
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Abstract
General methods and strains of bacteria are described that dramatically simplify and streamline plasmid DNA production. In one preferred embodiment, endolysin mediated plasmid extraction combined with flocculation mediated removal of cell debris and host nucleic acids achieves increased yield and purity with simplified downstream purification and reduced waste streams, thus reducing production costs.
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