Stem cell defined media for xeno-free and feeder free conditions and uses thereof

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United States of America Patent

PATENT NO 9725695
SERIAL NO

13696286

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Abstract

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The invention provides a defined low protein culture medium for maintaining cells in an undifferentiated state, the medium comprising: a basal medium, an organic acid from the tricarboxylic acid cycle, nonessential amino acids, a combination of growth factors selected from the group consisting of FGF-2 protein, an IGF-1 protein or insulin, a Transferrin protein, and a TGF beta 1 protein, wherein the medium is essentially feeder-free, essentially xeno-free, essentially free of beta-mercaptoethanol, and essentially free of animal-derived or human-derived proteins.

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Patent Owner(s)

  • THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;UNIVERSIDADE FEDERAL DO RIO DE JANEIRO

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Dos, Reis Castilho Leda Rio de Janeiro, BR 2 3
Marinho, Paulo Andre Nobrega La Jolla, US 1 3
Muotri, Alysson La Jolla, US 3 8
Rehen, Stevens Kastrup Rio de Janeiro, BR 3 3

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