Animal product-free culture medium and a process for producing a supernatant of clostridium comprising one or more collagenolytic and gelatinolytic proteases
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United States of America Patent
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Aug 8, 2017
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May 29, 2013
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May 31, 2012
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Abstract
The present invention refers to a culture medium and a process for producing Clostridium collagenolytic and gelatinolytic proteases. The present invention includes an animal product-free culture medium for C. histolyticum comprising non-animal derived peptones, preferably vegetable derived peptones, yeast extract and the amino acids cysteine and arginine. The present invention also includes a process for producing a supernatant of Clostridium histolyticum liquid culture comprising one or more collagenolytic and gelatinolytic proteases, and pharmaceutical composition comprising as active ingredient a supernatant of the Clostridium cultures described herein.
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