Methods for creating doubled haploid maize embryos using oil matrices
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United States of America Patent
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Jul 18, 2017
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Jul 7, 2015
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Aug 29, 2014
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Abstract
Methods for preserving viability of plant tissues such as plant embryos are provided herein. Also included are methods for storing genomic DNA and/or molecular marker assay materials in an oil bilayer as part of a high-throughput molecular characterization system. Moreover, plant embryos may be treated while in an oil matrix. The treatment may include chromosome doubling, Agrobacterium-mediated transformation, or herbicide selection as part of an embryo rescue process.
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