Purification method for embryo—derived infectious bronchitis virus (IBV)
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United States of America Patent
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Jul 11, 2017
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app pub date -
Nov 18, 2015
filing date -
Jan 30, 2015
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Abstract
The invention discloses an avian infectious bronchitis virus purification method. The method uses ordinary avian embryo which was affected by avian bronchitis virus. Using the combination of centrifugation, ultrafiltration, concentration, molecular sieve chromatography and ultrafiltration method can effectively remove miscellaneous protein in embryo-derived infectious bronchitis virus antigen, high virus recovery, and have no effect on the activity of avian infectious bronchitis virus. The avian infectious bronchitis virus purified by this method can be directly used in the preparation of avian infectious bronchitis virus vaccine.
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