Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate
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United States of America Patent
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Jun 27, 2017
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Jan 21, 2016
app pub date -
May 19, 2015
filing date -
Jun 17, 2008
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Abstract
A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.
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