NOV mini-promoters
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United States of America Patent
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Jun 27, 2017
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app pub date -
May 20, 2016
filing date -
May 21, 2015
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Abstract
Isolated polynucleotides comprising a NOV mini-promoter are provided. The mini-promoter may be operably linked to an expressible sequence, including, but not limited to, reporter genes, genes encoding a polypeptide of interest, and regulatory RNA sequences such as miRNA, siRNA, and anti-sense RNA. In some embodiments a cell comprising a stable integrant of an expression vector is provided, which may be integrated in the genome of the cell. The promoter may also be provided in a vector, for example in combination with an expressible sequence. The polynucleotides find use in a method of expressing a sequence of interest, including, but not limited to, for identifying or labeling cells, monitoring or tracking the expression of cells and gene therapy.
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