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United States of America Patent
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Jun 13, 2017
Grant Date -
Jun 19, 2014
app pub date -
Dec 19, 2013
filing date -
Dec 19, 2012
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Abstract
Disclosed is a method for producing a recombinant protein of interest, the method being characterized in by the following steps:
- (a) providing a fusion protein comprising an Npro autoprotease moiety and a protein of interest moiety in inclusion bodies,(b) solubilizing the inclusion bodies,(c) allowing the fusion protein to be cleaved by the Npro autoprotease moiety under chaotropic conditions, wherein the recombinant protein of interest is cleaved from the fusion protein and wherein the recombinant protein of interest is not yet renatured or simultaneously renatured, and(d) recovering the protein of interest, optionally including a renaturing step for the protein of interest.
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