Affinity tag system
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United States of America Patent
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Jun 13, 2017
Grant Date -
Jan 1, 2015
app pub date -
Jan 7, 2013
filing date -
Jan 6, 2012
priority date (Note) -
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Abstract
The present invention pertains to an affinity tag system for the immobilization and/or purification of molecules such as biological or organic molecules. The invention provides EF-hand subdomains of calcium binding proteins, such as calbindin D9k, as affinity tags and affinity ligands for immobilizing, detecting and/or for purifying molecules, particularly proteins. Also provided are methods utilizing the affinity tag system of the invention, affinity matrices comprising EF-hand subdomain affinity ligands and fusion proteins comprising EF-hand subdomain affinity tags.
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