Modified biotin, mutant streptavidin, and use thereof

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United States of America Patent

PATENT NO 9670255
APP PUB NO 20160137704A1
SERIAL NO

14768916

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Abstract

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It is an object of the present invention to provide a mutant streptavidin with a reduced affinity for natural biotin, and also to provide a modified biotin having a high affinity for the mutant streptavidin with a reduced affinity for natural biotin. According to the present invention, there is provided a reagent kit for use in treatments or diagnoses, which comprises: (a) a mutant streptavidin with a reduced affinity for natural biotin or biocytin; and a modified biotin having a high affinity for the above-described mutant streptavidin.

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Patent Owner(s)

Patent OwnerAddress
COMPLECURE INC1-12-8 SHIBUYA SHIBUYA-KU TOKYO 150-0002

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Doi, Hirofumi Chiba, JP 43 403
Inoue, Tsuyoshi Osaka, JP 156 1391
Kanai, Motomu Tokyo, JP 39 17
Kawato, Tatsuya Tokushima, JP 4 4
Kodama, Tatsuhiko Tokyo, JP 49 169
Meshizuka, Tomohiro Osaka, JP 3 4
Mizohata, Eiichi Osaka, JP 3 4
Shimizu, Yohei Tokyo, JP 57 186
Sugiyama, Akira Tokyo, JP 204 3358
Yamamoto, Tomohiro Tokyo, JP 84 2734

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