Red genetically encoded calcium indicators and methods of use
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United States of America Patent
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May 9, 2017
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app pub date -
Dec 18, 2015
filing date -
Dec 23, 2014
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Abstract
Protein indicators useful for calcium imaging, in particular, red genetically-encoded calcium indicators (GECIs) disclosed herein rival best-of-class green GECIs in terms of sensitivity for detecting neural activity, and can be monitored in vivo. The presently-disclosed subject matter further includes a method of monitoring cell activity comprising stimulating a cell comprising a red GECI polypeptide; and detecting fluorescence emitted by the cell.
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