Microfluidic device with reaction sites configured for blind filling
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United States of America Patent
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May 9, 2017
Grant Date -
Mar 14, 2013
app pub date -
Apr 4, 2012
filing date -
Nov 30, 2001
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Abstract
A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyzes. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyzes requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyzes.
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