Method for detecting methylated cytosine by using bisulfite reaction
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United States of America Patent
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Apr 18, 2017
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Dec 10, 2012
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Dec 14, 2011
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Abstract
The invention provides a method for converting non-methylated cytosine in a single-stranded DNA into uracil by a bisulfite reaction with a high conversion efficiency from non-methylated cytosine into uracil. The invention also provides a method for amplifying the single-stranded DNA in which non-methylated cytosine has been converted into uracil, as well as a method for detecting methylated cytosine in the single-stranded DNA.
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