Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps
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United States of America Patent
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Apr 11, 2017
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Aug 28, 2014
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Dec 22, 2010
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Abstract
The present invention relates to a method of using nanopores to obtain sequence information of sample DNAs in ss test DNAs. The method comprises using speed bumps to stall the ss test DNAs in the nanopores at random positions of the ss test DNAs to obtain sequence information of each and every nucleotides of the sample DNAs, and to construct the whole sequences of the sample DNAs. The present invention also relates to identification and/or isolation of test DNAs having desired sequence(s) using nanopore detectors facilitated by speed bump.
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