Method for reducing primer-dimer amplification
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United States of America Patent
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Mar 28, 2017
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Jan 12, 2017
app pub date -
Jun 16, 2016
filing date -
Jul 7, 2015
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Abstract
The present invention reduces primer-dimer amplification in a multiplex polymerase chain reaction (PCR). When a first forward primer (F1) and a second reverse primer (R2) have a complementary region at their 3′ends, primer dimers may form. The present method uses a primer comprising a 5′-end partial sequence or a full sequence of a first forward primer (F1^) in between a first tag (t1) and R2 to reduce the primer-dimer (F1_R2) amplification.
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