Method for purifying protein complexes
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United States of America Patent
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Mar 14, 2017
Grant Date -
Aug 25, 2011
app pub date -
Sep 7, 2009
filing date -
Sep 5, 2008
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Abstract
The present invention provides a method for the purification of complexes comprising a stress protein complexed to a peptide or peptide fragment, from a source mixture, typically a cell lysate. The improved method of the invention provides for protein complexes to be purified using ion exchange based methods, without the need to use chemicals such as chaotropes and ampholytes. The purified complexes can be used as the immunogenic determinant in vaccine compositions for the treatment or prevention of infectious diseases or cancerous conditions.
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