Bacterial host strain comprising a mutant spr gene and having reduced Tsp activity
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United States of America Patent
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Mar 7, 2017
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May 14, 2015
app pub date -
Jan 20, 2015
filing date -
Jan 14, 2010
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Abstract
The present invention provides a recombinant gram-negative bacterial cell comprising a mutant spr gene encoding a spr protein having a mutation at one or more amino acids selected from D133, H145, H157, N31, R62, 170, Q73, C94, S95, V98, Q99, R100, L108, Y115, V135, L136, G140, R144 and G147 and wherein the cell has reduced Tsp protein activity compared to a wild-type cell.
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