Method of selecting a monoclonal cell colony
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United States of America Patent
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Feb 28, 2017
Grant Date -
Sep 24, 2015
app pub date -
Sep 13, 2013
filing date -
Sep 14, 2012
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Abstract
A method of selecting a monoclonal cell colony that secretes a product of interest providing a plurality of cell culture spaces; each cell culture space has a respective assessment surface with a first binding agent immobilized on the assessment surface. The cells are incubated in a growth medium in the cell culture spaces for replication of the cells. At least some of the cells secrete a product of interest. A second binding agent is provided in the cell culture spaces. Initially, the second binding agent is not immobilized. Each of the first and second binding agents binds to the product of interest so that secretion of the product of interest by cells in any one of the cell culture spaces leads to a complex with the first binding agent, the product of interest and the second binding agent forming at the corresponding assessment surface of the cell culture space.
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