Detection of an amplification reaction product using pH-sensitive dyes
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United States of America Patent
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Feb 28, 2017
Grant Date -
Aug 27, 2015
app pub date -
Apr 7, 2015
filing date -
Aug 23, 2012
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Abstract
Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or PCR amplification reactions to completion using pH-sensitive dyes that are either colored or fluorescent. Compositions are described that include a mixture of a DNA polymerase, deoxyribonucleotide triphosphate and a weak buffer of less than 1 mM Tris or equivalent or no buffer.
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