Method for endotoxin removal
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United States of America Patent
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Feb 28, 2017
Grant Date -
Feb 5, 2015
app pub date -
Feb 27, 2013
filing date -
Feb 29, 2012
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Abstract
The present invention relates to a method for endotoxin removal from a sample comprising the following steps: combining the sample comprising one or more target molecule(s) with a chromatography media comprising beads having an inner porous core functionalized with ligands capable of binding endotoxin and an outer porous layer without functional groups and a pore size small enough to exclude the target molecule from the inner core; and collecting the sample from the media, wherein the sample comprises an endotoxin level which is at least 75% less, preferably 90% less, than before the removal and the yield of the target molecule is at least 75%.
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