Method for detecting variation of gene for non-diagnostic purpose based on fluorescence quenching and probe thereof
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United States of America Patent
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Feb 21, 2017
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Sep 18, 2014
app pub date -
Oct 17, 2012
filing date -
Oct 19, 2011
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Abstract
A method for detecting variation of gene based on fluorescence quenching quantification comprises: performing single base extension at a specific site of a gene to be detected with marked probes and marked dideoxyribonucleotide triphosphate; detecting fluorescence quenching values, and determining SNP of the gene to be detected. The present invention also provides an oligonucleotide probe for the method.
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