Method of detecting OXA-048 carbapenemase producing bacteria
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United States of America Patent
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Feb 7, 2017
Grant Date -
Jun 11, 2015
app pub date -
Jul 29, 2013
filing date -
Jul 27, 2012
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Abstract
A method of detecting and/or specifically identifying bacteria producing OXA-048-type carbapenemase in a biological sample, includes the steps of: (a) placing the biological sample likely to contain the bacteria in contact with a reaction medium including at least one chromogenic substrate to detect an enzymatic activity, and temocillin at a concentration equal to or greater than 150 mg/L, preferably between 200 and 500 mg/L, (b) incubating the sample in the medium to allow the bacteria to grow, and (c) detecting the strains corresponding to the OXA-48 carbapenemase producing bacteria. A culture medium as implemented in step (a) is also provided.
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