Methods for performing dry chemistry, lateral flow-reconstituted chromatographic enzyme-driven assays
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United States of America Patent
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Jan 31, 2017
Grant Date -
Jun 25, 2015
app pub date -
Nov 25, 2014
filing date -
Feb 24, 2003
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Abstract
Methods for performing dry chemistry, lateral flow-reconstituted chromatographic enzyme-driven assays are described. A combination of components necessary to elicit a specific enzyme reaction are predeposited as substrate in dry form together with ingredients necessary to produce a desired color upon occurrence of the desired reaction. The strip is equipped with a sample pad placed ahead of the substrate deposit in the flowstream, to which liquid sample is applied. The sample flows from the sample pad into the substrate zone where it immediately reconstitutes the dried ingredients while also intimately mixing with them and reacting with them at the fluid front. The fluid front moves rapidly into the final “read zone” wherein the color developed is read against predetermined color standards for the desired reaction. The assay in the format of the invention is faster and easier to perform than analogous wet chemistry assays.
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