Nucleic acid sequence segment for enhancing protein expression
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United States of America Patent
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Jan 24, 2017
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Sep 29, 2016
app pub date -
Aug 7, 2015
filing date -
Mar 27, 2015
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Abstract
An isolated or a purified nucleic acid sequence for enhancing expression levels of a protein of interest, in 5′ to 3′ direction, comprises: a cytomegalovirus (CMV) promoter; an eEF-1 intron repeat (eEF-1 IR); and a regular sequence, which comprises: at least one tag element, a fixable linker sequence, wherein the fixable sequence is TEV sequence; and a multiple cloning site (MCS). By means of the array of the specific promoter and eEF-1 IR, the expression and purity of the recombinant protein could be enhanced; wherein eEF-1 IR can reduce the length of vector and assist RNA polymerase II transcription. Besides, TEV sequence of the fixable linker sequence of the regular sequence can remove a tag on recombinant protein; a specific target can be inserted into the multiple cloning site.
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