Supports for cell culture and cell sheet detachment and methods for cell sheet detachment
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United States of America Patent
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Jan 17, 2017
Grant Date -
Dec 6, 2012
app pub date -
May 31, 2011
filing date -
May 31, 2011
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Abstract
The invention creates a support for cell culture and cell sheet detachment which has a substrate, whose surface is coated with a conjugate having a disulfide bond-containing amino acid as a spacer and a biopolymer enhancing cell attachment, migration or differentation. Unexpectedly, after being seeded on the support, the cells grow to form one or more layers of cell sheets and the cell sheets can be easily detached from the support by adding a reductant to cleave the disulfide bond. Accordingly, the invention provides a simple and non-toxic method for detachment of cell sheets.
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