Microfluidic devices and methods of use in the formation and control of nanoreactors
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United States of America Patent
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Jan 3, 2017
Grant Date -
Oct 30, 2014
app pub date -
Apr 9, 2014
filing date -
Jan 11, 2006
priority date (Note) -
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Abstract
The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.
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International Classification(s)
- C12Q:MEASURING OR TESTING PROCESSES INVOLVING ENZYMES O....
- B82Y:SPECIFIC USES OR APPLICATIONS OF NANO-STRUCTURES; ....
- C12N:MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF
- G01N:INVESTIGATING OR ANALYSING MATERIALS BY DETERMININ....
- B01L:CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENE....
- B01J:CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, CO....
- C40B:COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL ....
- B01F:MIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
- C07K:PEPTIDES
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