Method of detecting residual genomic DNA and a kit thereof
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United States of America Patent
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Dec 6, 2016
Grant Date -
Jan 29, 2015
app pub date -
Mar 6, 2012
filing date -
Jan 16, 2012
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Abstract
The present disclosure relates to a highly specific and sensitive method of detecting host cell impurities in a biological sample by using quantitative real time polymerase chain reaction (q PCR). The present disclosure also provides novel designed primer and probe to amplify only the specific Alu family of dispersed repetitive sequences from Chinese hamster ovary cells used for expression of therapeutic proteins.
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