Activity of Fe—S cluster requiring proteins
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United States of America Patent
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Dec 6, 2016
Grant Date -
Feb 6, 2014
app pub date -
Mar 15, 2013
filing date -
Feb 17, 2010
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Abstract
The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
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