Regulatable promoter
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
Dec 6, 2016
Grant Date -
Aug 28, 2014
app pub date -
Oct 5, 2012
filing date -
Oct 7, 2011
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.
First Claim
Other Claims data not available
Family
International Classification(s)
- 2012 Application Filing Year
- C12P Class
- 1680 Applications Filed
- 1225 Patents Issued To-Date
- 72.92 % Issued To-Date
Cited Art Landscape
Patent Citation Ranking
- 1 Citation Count
- C12P Class
- 54.95 % this patent is cited more than
- 9 Age
Maintenance Fees
| Fee | Large entity fee | small entity fee | micro entity fee | due date |
|---|---|---|---|---|
| 11.5 Year Payment | $7400.00 | $3700.00 | $1850.00 | Jun 6, 2028 |
