Process for purification of a fatty acid binding protein
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United States of America Patent
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Oct 25, 2016
Grant Date -
Feb 25, 2016
app pub date -
Oct 19, 2015
filing date -
Sep 17, 2010
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Abstract
A process for purification of a fatty acid binding proteins such as, e.g., Sm14 of Pichia pastoris or type-3 FABP protein of Fasciola hepatica. The process includes the steps of: (a) performing lysis of cells containing the fatty acid binding protein to obtain a lysate; (b) clarifying the lysate obtained in step (a) to obtain a clarified lysate; (c) loading the clarified lysate in a column containing an anion exchange resin; (d) eluting proteins from the column by pH changes in the column; and (e) separating contaminant proteins from the fatty acid binding protein by gel-filtration.
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