Immunosorbent and immunoaffinity column for aflatoxin M1 nanobody and preparation method thereof

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United States of America Patent

PATENT NO 9435776
APP PUB NO 20150276729A1
SERIAL NO

14667378

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Abstract

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An aflatoxin M1 nanobody, an immunosorbent and an immunoaffinity column. The aflatoxin M1 nanobody 2014AFM-G2 has the amino acid sequence of SEQ ID NO:7, is encoded by the nucleic acid sequence of SEQ ID NO:8, has a 50% inhibiting concentration IC50 to aflatoxin M1 of 0.208 ng/mL, and has cross reaction rates with aflatoxins B1, B2, G1, and G2 of 9.43%, 5.93%, 4.87% and 6.17%, respectively. The immunosorbent includes a solid phase carrier and aflatoxin M1 nanobody 2014AFM-G2 coupled with the solid phase carrier. The immunoaffinity column is loaded with the aflatoxin M1 nanobody immunosorbent. It can be used for purifying and concentrating an extracting solution of a sample before loading to a machine for detection and the immunoaffinity column can be used repeatedly for many times.

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Patent Owner(s)

Patent OwnerAddress
OILCROPS RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURE SCIENCESWUCHANG WUHAN HUBEI 430062

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Ding, Xiaoxia Hubei, CN 13 6
He, Ting Hubei, CN 132 1503
Li, Peiwu Hubei, CN 32 10
Zhang, Qi Hubei, CN 768 6016
Zhang, Zhaowei Hubei, CN 13 3

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