Method and kit for purifying nucleic acids
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United States of America Patent
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Aug 30, 2016
Grant Date -
Jan 16, 2014
app pub date -
Aug 27, 2013
filing date -
Oct 31, 2007
priority date (Note) -
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Abstract
Methods for automated extraction of nucleic acids are disclosed. Also disclosed are method and kits for isolating fetal nucleic acids from a plasma sample of a pregnant woman. The method includes flowing the plasma sample through a first filter under conditions that allow binding of the fetal and maternal nucleic acids to the first filter; eluting the fetal and maternal nucleic acids bound to the first filter to produce a concentrated nucleic acid sample; flowing the concentrated nucleic acid sample through a second filter under conditions that allow preferential binding of the maternal nucleic acids to the second filter; and recovering the fetal nucleic acid from the concentrated nucleic acid sample that flow through the second filter.
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