Endonucleases
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United States of America Patent
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Aug 23, 2016
Grant Date -
Dec 18, 2014
app pub date -
Feb 18, 2013
filing date -
Feb 17, 2012
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Abstract
The present invention provides an endonuclease I or enzymatically active fragment thereof wherein said endonuclease I has the sequence of SEQ ID No. 4 or a sequence which is at least 70% identical thereto and wherein the amino acid residue which is immediately N-terminal of the FYCGC pentapeptide motif has been substituted with a residue which is negatively charged as well as nucleic acid molecules encoding these enzymes and methods of removing contaminating polynucleotides from a sample using these enzyme.
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