Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
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United States of America Patent
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Aug 23, 2016
Grant Date -
Feb 23, 2012
app pub date -
May 18, 2011
filing date -
May 19, 2010
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Abstract
Methods and materials for determining the presence of at least one nucleic acid in a sample are provided, said methods comprising (1) a purification step using sequence specific hybrid capture; (2) an amplification step; and (3) a detection step comprising contacting the target nucleic acid with a plurality of detectably labeled nucleic acid detection probes, wherein each (a) bears a different detectable label from the other detection probes, and/or (b) has a different melting temperature from probes bearing the same detectable label. Also disclosed are compositions and kits for use in such a method.
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