Method for enhancing catalytic activity of a lipase
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United States of America Patent
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Aug 16, 2016
Grant Date -
Dec 30, 2010
app pub date -
Apr 28, 2008
filing date -
Apr 27, 2007
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Abstract
Methods for enhancing a biological activity, for example, catalytic activity, of a lipase, are provided. In some embodiments, the methods include the step of alkylating one or more cysteine residues present within the lipase. Also provided are modified polypeptides for which a biological activity is enhanced by the disclosed methods, methods for using the disclosed polypeptides, including for the transesterification of renewable oils to produce a biofuel, and cell free systems that include a lipase, to which one or more moieties, such as steroidal moieties, are conjugated.
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