Method for the production of polypeptides
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United States of America Patent
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Aug 16, 2016
Grant Date -
Feb 12, 2015
app pub date -
Mar 18, 2013
filing date -
Mar 19, 2012
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Abstract
New methods for the production of recombinant polypeptides from inclusion bodies are disclosed. Modulation of the cell culture conditions positively affects the yield of the recombinant polypeptide in active form. In one aspect, the methods comprise (a) cultivating a host cell at a first temperature, the host cell comprising a nucleic acid encoding a recombinant polypeptide, (b) lowering the cultivation temperature from the first temperature to a second temperature, and (c) cultivating the host cell at the second temperature.
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