Production of isopropanol by improved recombinant strains
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United States of America Patent
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Jul 26, 2016
Grant Date -
Oct 30, 2014
app pub date -
Oct 3, 2012
filing date -
Oct 11, 2011
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Abstract
The expression vector includes: the nucleic acids coding for the polypeptides forming a polypeptide complex having an enzyme activity allowing acetoacetyl-CoA to be converted to acetoacetate; optionally, at least one nucleic acid coding for a polypeptide having an enzyme activity allowing acetoacetate to be converted to acetone; and at least one nucleic acid coding for a polypeptide having an enzyme activity allowing acetone to be converted to isopropanol; the expression of the nucleic acids being controlled by a single constitutive promoter located upstream of the abovementioned nucleic acids.
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