Method and apparatus for precise selection and extraction of a focused component in isoelectric focusing performed in micro-channels
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United States of America Patent
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Jun 28, 2016
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Jan 23, 2014
app pub date -
Oct 3, 2012
filing date -
Jul 10, 2006
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Abstract
An apparatus and method are disclosed for the precise selection and extraction of a selected analyte in a focused zone produced by isoelectric focusing performed in micro-channels. A cross-channel microfluidic device comprises a sample mixture introduction and separation channel and an extraction channel, which are in fluid communication with each other at a point of intersection. Means are provided for selectively moving the pattern of separated zones following cIEF to the intersection point, and means are provided for applying an extraction pressure to direct a single zone containing a selected analyte into and then out of the extraction channel for collection.
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