Glucose dehydrogenase/cytochrome fusion protein
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United States of America Patent
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May 31, 2016
Grant Date -
Sep 19, 2013
app pub date -
Oct 19, 2012
filing date -
Sep 30, 2003
priority date (Note) -
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Abstract
A fusion protein of pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) and a cytochrome is disclosed. PQQGDH is, for example, a water-soluble PQQGDH derived from Acinetobacter calcoaceticus. The cytochrome is, for example, an electron transfer domain of quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni. The fusion protein of the present invention shows intramolecular electron transfer from PQQ, a redox center, to the cytochrome, which allow construction of a direct electron transfer-type glucose sensor which requires no electron mediators.
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