Method for collecting nucleated red blood cells via density-gradient centrifugation utilizing changes in blood cell density
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United States of America Patent
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May 10, 2016
Grant Date -
Mar 21, 2013
app pub date -
Feb 21, 2011
filing date -
Aug 20, 2010
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Abstract
Method for concentrating and collecting small quantities of fetal nucleated red blood cells contained in the maternal blood. The method for concentrating and collecting nucleated red blood cells from the maternal blood comprises: (i) subjecting the maternal blood to a first density-gradient centrifugation and collecting a cell fraction containing nucleated red blood cells; (ii) treating the cell fraction containing nucleated red blood cells so as to selectively changes the density of the nucleated red blood cells from that of the white blood cells; and (iii) subjecting the treated cell fraction containing the nucleated red blood cells to a second density-gradient centrifugation so as to collect a fraction containing nucleated red blood cells.
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