System and method to correct out of phase errors in DNA sequencing data by use of a recursive algorithm
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United States of America Patent
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Apr 19, 2016
Grant Date -
May 23, 2013
app pub date -
Dec 18, 2012
filing date -
Feb 16, 2006
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Abstract
Provided herein are methods for correcting an error associated with phasic synchrony of sequence data generated from a population of template molecules by detecting signals generated in response to nucleotide species introduced during a sequencing reaction; generating an observed value for the signal detected from each of the nucleotide species; defining positive incorporation values and negative incorporation values from the observed values using a carry forward value and an incomplete extension value; revising the carry forward value and the incomplete extension value using a noise value derived from observed values associated with the negative incorporation values; re-defining the positive incorporation values and the negative incorporation values using the revised carry forward value and the revised incomplete extension value; and repeating the steps of revising and re-defining until convergence of the positive incorporation values and the negative incorporation values.
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