Gene targeting vector, and method for using same
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United States of America Patent
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Apr 5, 2016
Grant Date -
Nov 13, 2014
app pub date -
Dec 12, 2012
filing date -
Dec 13, 2011
priority date (Note) -
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Abstract
Provided is a gene targeting vector that enables highly efficient gene targeting. The gene targeting vector has a structure comprising a positive selection marker flanked by a DNA homologous to a 5′-upstream region of a target site and a DNA homologous to a 3′-downstream region of the target site, wherein a splice acceptor site and a DNA sequence allowing for bicistronic expression are added 5′-upstream of the positive selection marker, and another splice acceptor site is also added 5′-upstream of the DNA homologous to the 5′-upstream region of the target site.
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