Intein-modified enzymes, their production and industrial application

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United States of America Patent

PATENT NO 9303250
APP PUB NO 20130247251A1
SERIAL NO

13777166

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Abstract

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A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.

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Patent OwnerAddress
AGRIVIDA INC78E OLYMPIA AVENUE WOBURN MA 01801

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Apgar, James Somerville, US 10 48
de, la Vega Humberto Peabody, US 12 48
Lazar, Gabor Belmont, US 14 46
Lessard, Philip Framingham, US 6 38
Raab, R Michael Arlington, US 42 173
Shen, Binzhang Boston, US 14 46

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