Quantitative multiplexed identification of nucleic acid targets
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United States of America Patent
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Mar 15, 2016
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May 24, 2012
app pub date -
Oct 14, 2011
filing date -
Oct 14, 2010
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Abstract
Methods and systems for detecting a target nucleic acid using the quantitative capabilities of real-time nucleic acid amplification systems and the multiplexing capabilities of hybridization systems, comprising: identifying a conservative sequence and a distinctive sequence within each target nucleic acid sequence; simultaneously amplifying the conservative region and the distinctive region; monitoring the amplification of the conservative region in real-time; identifying the distinctive region amplicon via multiplexed identification; and performing quantitative multiplexing analysis of the target by combining the real-time monitoring information with the multiplexed identification of the target nucleic acid.
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