Compositions and methods for in vitro diagnostic tests including sulfonic acid compound
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United States of America Patent
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Jan 5, 2016
Grant Date -
Sep 26, 2013
app pub date -
Mar 22, 2013
filing date -
Mar 23, 2012
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Abstract
The invention provides compositions, kits, and methods for performing colorimetric analysis. A substrate is reacted to generate a chromogenic reaction product, and a reaction stop reagent that is a sulfonic acid is added to stop and stabilize the reaction product. The absorbance properties of the chromogenic reaction product can be maintained over significantly longer periods of time of that of conventional reagents and methods. The sulfonic acid can be used in assays such as ELISAs in order to provide a more accurate and safer detection of analytes in a biological sample.
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