Method and kit for detecting hepcidin
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United States of America Patent
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Nov 24, 2015
Grant Date -
Aug 7, 2014
app pub date -
Jun 1, 2012
filing date -
Jun 3, 2011
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Abstract
A method for detecting hepcidin. Having a sample liquid in contact with a nanochip having a specific surface coating structure of silicon oxide, so that hepcidin is enriched with specificity; eluting the nanochip with an eluent; by performing mass spectrometric detection on the elution product, determining the hepcidin content in the elution product. The enrichment method substantially enhances the sensitivity and accuracy of mass spectrometric detection. A kit for detecting hepcidin comprising a sample diluent and a nanochip, the sample diluent comprising water, trifluoroacetic acid, and acetonitrile.
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